Abstract: Hydroxyapatite (HAP) scaffold and hydroxyapatite/chitosan (HAP-CS) composite scaffold mixed with chitosan were prepared using three dimensional printing (3DP) manufacturing technology. And HAP-CS scaffold was modified with Collagen TypeⅠ. Properties of the prepared scaffolds have been studied through compressive strength experiment，porosity measurement，micro morphology observation，hydrophily and alkaline phosphatase (ALP) activity experiment. The results demonstrated that CS could result in a 74.5% improvement of compressive strength of HAP scaffold，while the porosity of scaffold was not decreased. The hydrophily of HAP-CS scaffold modified with Collagen Type Ⅰ was significantly enhanced. ALP activity experiment results indicated that Collagen Type Ⅰ could improve the ossification ability of cell on the HAP-CS scaffold. Excellent structural properties and biological activity have been observed from the HAP-CS scaffold modified with Collagen Type Ⅰ ，which could be used for the research of bone defect repair.

Key words: three dimensional printing, scaffold, chitosan, collagen type I

摘要： 利用三维打印（3DP）成型技术制备了羟基磷灰石（HAP）支架与混合壳聚糖（CS）的HAP-CS复合支架，并用Ⅰ型胶原蛋白（Collagen TypeⅠ）对HAP-CS支架进行了改性处理；通过抗压强度实验、孔隙率测定、微观形貌观察、亲水性及碱性磷酸酶（ALP）活性检测实验研究了所制备支架性能。结果表明添加CS可提高HAP支架抗压强度74.5%，但未降低支架孔隙率；经Collagen Type I改性的HAP-CS支架的亲水性有显著提高，ALP活性检测结果显示Collagen Type Ⅰ可提高细胞骨化能力。综上，本研究所制备的HAP-CS改性支架具有良好的性能及生物活性，可用于骨缺损修复研究。

关键词: 三维打印, 支架, 壳聚糖, Ⅰ型胶原蛋白