Chemical Industry and Engineering Progree

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Preparation of pH sensitive fluorescent-labeled water-soluble chitosan

XIE Qinqin,XIN Meihua,LI Mingchun,MAO Yangfan   

  1. College of Material Science and Engineering,Huaqiao University,The Key Laboratory for Functional Materials of Fujian Higher Education,Xiamen 361021,Fujian,China
  • Online:2010-10-05 Published:2010-10-05

pH响应水溶性荧光标记壳聚糖的制备及表征

谢钦钦,辛梅华,李明春,毛扬帆   

  1. 华侨大学材料科学与工程学院,福建省高校功能材料重点实验室,福建 厦门 361021

Abstract:

A fluorescent-labeled water-soluble chitosan derivative PyHy-TMCMC was preparated by modifying chitosan and characterized by FTIR1H NMR and fluorescence spectroscopy. The relationship between pH and fluorescence of PyHy-TMCMC was studied. Result showed that the fluorophore was successfully labeled to chitosan and the fluorescence intensity of PyHy-TMCMC was sensitive in the pH 114 rangewhich would potentially be used as a pH sensitive fluorescence probe.

摘要:

通过对壳聚糖进行三甲基化和羧甲化改性,制备了可溶于水的荧光标记壳聚糖衍生物芘丁酰肼-三甲基羧甲化壳聚糖,产物用FTIR1H NMR和荧光光谱进行表征,并试验了介质pH值对其荧光性能的影响。结果表明,在pH114水溶性芘丁酰肼-三甲基羧甲化壳聚糖具有pH响应性,可望用作pH荧光探针高分子材料。

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