Abstract:

A fluorescent-labeled water-soluble chitosan derivative PyHy-TMCMC was preparated by modifying chitosan and characterized by FTIR，¹H NMR and fluorescence spectroscopy. The relationship between pH and fluorescence of PyHy-TMCMC was studied. Result showed that the fluorophore was successfully labeled to chitosan and the fluorescence intensity of PyHy-TMCMC was sensitive in the pH 1—14 range，which would potentially be used as a pH sensitive fluorescence probe.

摘要：

通过对壳聚糖进行三甲基化和羧甲化改性，制备了可溶于水的荧光标记壳聚糖衍生物芘丁酰肼-三甲基羧甲化壳聚糖，产物用FTIR、¹H NMR和荧光光谱进行表征，并试验了介质pH值对其荧光性能的影响。结果表明，在pH值1～14时水溶性芘丁酰肼-三甲基羧甲化壳聚糖具有pH响应性，可望用作pH荧光探针高分子材料。