Enrichment of PHAs-producing bacteria by granular sludge in ABR acidogenic sulfate-reducing phase

doi:10.16085/j.issn.1000-6613.2023-0203

Abstract

Abstract:

In order to investigate the feasibility of synthesizing polyhydroxyalkanoates (PHAs) from sulfate organic wastewater through anaerobic treatment, a five-compartment anaerobic baffled reactor (ABR) was utilized to enrich PHAs-producing bacteria using sulfate organic wastewater as substrate, and the effects of different influent COD/SO₄^2- ratios (12.5, 9.3 and 4.0) on PHAs synthesis efficiency of granular sludge in the acidogenic sulfate-reducing phase (the first and second compartments) of ABR were examined to explore the PHA synthesis pattern in this system. The results showed that as the inflow COD/SO₄^2- ratio decreased, the removal of COD and SO₄^2- in the acidogenic sulfate-reduction phase shifted downstream. In the first compartment, the dominant microbial metabolism type was the butyric acid type, while in the second compartment, it shifted from the acetic acid type to the butyric acid type. The compartment with high content of PHAs in the granular sludge shifted from the first compartment to the second compartment. The best PHAs synthesis effect was observed when the inflow COD/SO₄^2- ratio was 9.3, which led to a large enrichment of PHAs-producing bacteria and high production of PHAs in the granular sludge of the acidogenic sulfate-reduction phase. The PHAs-producing bacteria in the granular sludge were large, and the PHAs granules were densely distributed throughout the bacterial cells. In the acidogenic sulfate-reducing phase, there existed a bio-chain cooperation relationship among acid-producing bacteria (APB), fatty-acid utilized sulfate reducing bacteria (FSRB) and acetic-acid utilized sulfate reducing bacteria (ASRB), and a large amount of PHAs was formed inside the bacterial cells. This study provided theoretical support for the resource utilization of sulfate organic wastewater.

Key words: polyhydroxyalkanoates, anaerobic baffled reactor, acidogenic sulfate-reducing phase, metabolic type, synthesis pathway

摘要：

为探究硫酸盐有机废水厌氧处理合成聚羟基脂肪酸酯（PHAs）的可行性，利用五隔室厌氧折流板反应器（ABR）以硫酸盐有机废水为底物富集PHAs产生菌合成PHAs，考察不同进水COD/SO₄^2-比值（12.5、9.3、4.0）对ABR产酸-硫酸盐还原相（第1、第2隔室）颗粒污泥PHAs合成效果的影响，进而探讨该体系中PHAs合成模式。结果表明：随进水COD/SO₄^2-比值降低，产酸-硫酸盐还原相的COD与SO₄^2-去除沿程后移，第1隔室呈现丁酸型代谢类型为主，第2隔室由乙酸型转为丁酸型代谢类型为主；颗粒污泥PHAs的高含量隔室由第1隔室后移至第2隔室，其中COD/SO₄^2-比值为9.3时，产酸-硫酸盐还原相中颗粒污泥PHAs产生菌大量富集、PHAs合成效果最好；在颗粒污泥中PHAs产生菌个体大，PHAs颗粒密集地布满整个菌体细胞；产酸-硫酸盐还原相中存在产酸菌（APB）、脂肪酸型硫酸盐还原菌（FSRB）、乙酸型硫酸盐还原菌（ASRB）生物链式协同代谢模式，并且在菌体内形成大量PHAs。该研究为硫酸盐有机废水的资源化利用提供理论支持。

关键词: 聚羟基脂肪酸酯, 厌氧折流板反应器, 产酸-硫酸盐还原相, 代谢类型, 合成途径

CLC Number:

X703

WANG Chenxiang, QIN Yongli, JIANG Yongrong, GE Shijia, ZHENG Guoquan, SUN Zhenju. Enrichment of PHAs-producing bacteria by granular sludge in ABR acidogenic sulfate-reducing phase[J]. Chemical Industry and Engineering Progress, 2023, 42(12): 6658-6665.

汪晨祥, 秦永丽, 蒋永荣, 葛仕佳, 郑国权, 孙振举. ABR产酸-硫酸盐还原相颗粒污泥富集PHAs产生菌[J]. 化工进展, 2023, 42(12): 6658-6665.

Figures/Tables 8

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VFAs	COD/SO₄^2-=12.5		COD/SO₄^2-=9.3		COD/SO₄^2-=4.0
VFAs	第1隔室	第2隔室	第1隔室	第2隔室	第1隔室	第2隔室
乙酸/mg·L^-1	414(29%)	894(69%)	462(28%)	1056(73%)	420(22%)	696(34%)
丙酸/mg·L^-1	215(15%)	140(11%)	251(15%)	96(7%)	200(11%)	185(9%)
丁酸/mg·L^-1	659(46%)	255(20%)	774(48%)	299(20%)	994(54%)	941(45%)
乳酸/mg·L^-1	135(10%)	未检出	144(9%)	未检出	234(13%)	243(12%)
总和/mg·L^-1	1423	1289	1631	1451	1848	2065

VFAs	COD/SO₄^2-=12.5		COD/SO₄^2-=9.3		COD/SO₄^2-=4.0
VFAs	第1隔室	第2隔室	第1隔室	第2隔室	第1隔室	第2隔室
乙酸/mg·L^-1	414(29%)	894(69%)	462(28%)	1056(73%)	420(22%)	696(34%)
丙酸/mg·L^-1	215(15%)	140(11%)	251(15%)	96(7%)	200(11%)	185(9%)
丁酸/mg·L^-1	659(46%)	255(20%)	774(48%)	299(20%)	994(54%)	941(45%)
乳酸/mg·L^-1	135(10%)	未检出	144(9%)	未检出	234(13%)	243(12%)
总和/mg·L^-1	1423	1289	1631	1451	1848	2065

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