Abstract: This research used a single chamber microbial electrolysis cell（MEC）as the reactor to treat the pretreated fermentation effluent rich in acetic acid to produce hydrogen. The accumulated acetic acid was chosen as the main electron donor，with carbon cloth as the anode and the stainless steel wire mesh coated with Ni nanoparticles as the catalyst. The experimental results showed that in the MEC fed by the fermentation effluent reached a maximum hydrogen yield of (1.31±0.04) m3H2/(m3?d) and (2.78±0.11) mLH2/mgCOD，and energy efficiency of 138.6%±3.1% with COD removal of 99.0%±0.3%. This research also revealed that MEC could degrade the liquid end-products spontaneously and decrease the “fermentation barrier” to realize production control and energy product.

Key words: acetic acid, fermentation barrier, microbial electrolysis cell (MEC), bio-hydrogen production

摘要： 采用单室微生物电解池（MEC）反应器为实验装置，通过预处理技术强化发酵制氢废液中乙酸的积累，并将该发酵废液作为底物，考察了以废液中累积的乙酸作为主要电子供体、碳布为阳极、涂布有Ni纳米颗粒的不锈钢网为催化阴极的产氢效果。结果表明，在MEC中，以预处理的发酵制氢废液积累的乙酸为底物，最高产氢率可达(1.31±0.04) m3H2/(m3?d)和(2.78±0.11) mLH2/mgCOD，同时可获得138.6%±3.1%的能量效率和99.0%±0.3%的COD去除率。实验表明，利用MEC可将发酵末端产物进一步降解，从而减弱了“发酵障碍”现象，实现了治污和产能的统一。

关键词: 乙酸, 发酵障碍, 微生物电解池, 生物制氢