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Utilization of feruloyl esterase and xylanase for the degradation of brewers’ spent grain

LI Xialan1,CHENG Shanying1,YANG Daoxiu1,FANG Baishan1,2   

  1. 1 Department of Chemical and Biochemical Engineering,Institute of Chemical Technology,Huaqiao University, Xiamen 362021,Fujian,China;2 Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,Fujian,China
  • Online:2012-05-05 Published:2012-05-05

阿魏酸酯酶和木聚糖酶协同降解麦糟

李夏兰1,程珊影1,杨道秀1,方柏山1,2   

  1. 1华侨大学化工学院生物工程系,福建 厦门 361021;2厦门大学化学化工学院化学工程与生物工程系,福建 厦门 361005

Abstract: The optimal fermentation time of Penicillium citrinum feruloyl esterase(PcFAE)was 60 h and its activity was 40.8 mU/mL when the Brewers’ Spent Grain(BSG)was used as the sole carbon source for Penicillium citrinum. One gram of alcohol-insoluble residue(AIR)of Brewers’spent grain(BSG)was processed by the solution of crude xylanase(XG180)(47.5 U/mL,15 mL)for 12 hours at the condition of the material-liquid ratio 1∶30(W/V),pH 5.0 and 45 ℃,respectively. And then the solution of crude PcFAE(40.8 mU/mL)was added and the sample was processed for another 12 h. The maximum release rate of Ferulic acid(FA)was 54.1% and the release amount of xylooligosaccharide(XOS)was 161 mg/g(BSG-AIR),respectively. The results of our experiments showed that the release rate of FA and XOS from BSG-AIR increased significantly as the PcFAE could coordinate with the xylanase and enzymolysis process was also conducive to the degradation of BSG.

Key words: feruloyl esterases;xylanase;brewers&rsquo, spent grain;biodegradation

摘要: 橘青霉以麦糟为唯一碳源培养时,阿魏酸酯酶的最佳发酵时间为60 h,其酶活力可达40.8 mU/mL。在pH值为5.0、45 ℃、料液比1∶30(g∶mL)条件下,取47.5 U/mL的木聚糖酶粗酶液15 mL,加入1.0 g 麦糟的乙醇不溶物,反应12 h后,加入40.8 U/mL的阿魏酸酯酶粗酶液15 mL再反应12 h,阿魏酸和低聚木糖释放率分别为54.1%和161 mg/g (麦糟的乙醇不溶物)。实验结果还表明,阿魏酸酯酶与木聚糖酶存在协同作用,能极大提高麦糟中阿魏酸及低聚木糖的释放率,有利于麦糟的降解。

关键词: 阿魏酸酯酶, 木聚糖酶, 麦糟, 生物降解

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