Abstract:

Isomaltulose is a functional sweetener emerged in recent years and has wide application prospects in the food and chemical industries. Sucrose isomerase（SIase）is currently the most effective enzyme for isomaltulose industrial production. The research progress of the SIase producing strain，purification and characterization as well as protein structure and function is reviewed. The catalytic mechanism of SIase for isomaltulose and trehalulose production，and the application in isomaltulose production are also presented. The research perspectives of SIase are discussed，and the research emphasis should be placed on screening ideal microbe strains and fermentation process optimization to enhance SIase production.

摘要：

异麦芽酮糖是近年来新兴的一种功能性甜味剂，在食品、医药等工业中具有广阔的应用前景。蔗糖异构酶是生物法工业化生产异麦芽酮糖最有效的酶。本文介绍了近年来国内外蔗糖异构酶的生产菌株、酶学性质、结构与功能，阐述了蔗糖异构酶催化生产异麦芽酮糖和海藻酮糖的机制及其在异麦芽酮糖生产中的应用。并展望了其今后的研究方向：应从SIase高产菌株的选育、发酵产酶工艺优化方面开展工作，大幅度提高产酶水平。