Abstract: A method is described for successive purification of astaxanthin by medium-pressure silica gel column chromatography from Phaffia rhodozyma containing 20% astaxanthin at least. The column chromatography was performed by using 120—160 μm silica gel as filling material，petroleum ether-dichloromethane-acetone with 5∶2.5∶1 volume ratio as eluting solvent and the loading amount of 1∶7. The purity of obtained astaxanthin was higher than 97% and the average yields was 60% at least. The reclaimed eluting solvent can be used repeatedly，and the chromatography column can be used repeatedly through regeneration by acetone and equilibrated with petroleum. Compared with traditional method for astaxanthin purification，the current method has the advantages of less solvent usage，cheaper filling material，short production cycle and effective purification.

摘要： 采用连续中压硅胶柱层析纯化虾青素，原料为含量高于20%的法夫酵母虾青素。连续中压柱层析分离工艺条件为：不锈钢中压层析柱装填120～160 mm层析硅胶，以石油醚∶1,2-二氯乙烷∶丙酮（体积比）为5∶2.5∶1作洗脱剂，负载量为1∶7，可以得到纯度大于97%的虾青素产品，平均回收率大于60%。回收的洗脱液经校正后可重复使用。使用后的层析柱以丙酮为再生液再生，石油醚为平衡液平衡，平衡后的层析柱可重复使用多次。利用该工艺方法纯化虾青素，与传统方法相比溶剂用量少、层析填料价格低、生产周期短、分离效果好，易于实现连续工业化清洁生产。