Abstract: Biodenitrification is an effective way to deal with the nitrogen pollution of water bodies. In this paper，an aerobic denitrifier H1 was cultured by using (NH4)2SO4 as the nitrogen source and sodium citrate as the carbon source. After 4d of aerobic denitrification，NH4+-N removal rate reached to 76.92% and COD removal rate reached to 84.29%，indicating that H1 was a heterotrophic nitrification-aerobic denitrifier. When NH4+-N and NO3－-N existed as the nitrogen source together，NH4+-N removal rate reached to higher than 80% after 2d，while NO3－-N removal was lagged apparently，indicating H1 took use of NH4+-N as the nitrogen source preferentially. When H1 was used to deal with domestic sewage，the rapid conversion of organic nitrogen to ammonia nitrogen was observed. The total nitrogen，NH4+-N and COD removal rate could all reach to higher than 90%，indicating that H1 possess great application prospect in the field of sewage treatment.

摘要： 生物脱氮是目前处理水体氮素污染的有效方法，本文以(NH4)2SO4为氮源、柠檬酸三钠为碳源培养好氧反硝化菌H1，4天后NH4+-N的去除率达到76.92%，COD去除率达到84.29%，说明H1为异养硝化-好氧反硝化菌。当NH4+-N与NO3－-N同时存在时，H1对NH4+-N的去除率在2天后即达到80%以上，但对NO3－-N的去除明显滞后，说明H1优先利用NH4+-N。利用H1处理生活污水，其能够促使污水中的有机氮迅速转化为氨氮，最终使污水中总氮、NH4+-N、COD的去除率均达到90%以上，表明H1在生活污水处理领域具有巨大的应用前景。