Abstract: Response surface methodology（RSM）was used to optimize the process of homogenized extraction of five main phenol glycosides and aglycones（target products）from the root of Acanthopanax senticosus（Rupr. Et Maxim.）Harms. According to Box-Behnken experiment design，four independent variables were selected，i.e. ethanol volume fraction，extracting time，homogenization power and liquid to solid ratio，to investigate the influence on extraction efficiency. Meanwhile，extraction rate and the purity of three target products were chosen as responses. The optimal parameters were obtained as：extraction time of 4.04min，ethanol volume fraction of 59.66%，homogenization velocity of 11 999.6 r/min，liquid to solid ratio of 7.96∶1 mL/g and 3 times. Under the optimized condition，the extraction rate and purity of three target products were found as 0.001 6% and 0.052% for syringin，0.018% and 0.68% for isofraxidin，0.035% and 1.10% for eleutheroside E，respectively.

摘要： 采用响应面法对匀浆提取刺五加根茎原料中紫丁香苷、刺五加苷E和异秦皮啶等主要酚苷及苷元成分进行了优化。考察了匀浆提取时间、乙醇体积分数、匀浆转数和液料比等因素对提取工艺的影响，应用了Box-Behnken的实验设计，选取了3种目标产物的得率与纯度作为响应值。响应面法优化后提取工艺条件为提取时间4.04 min、乙醇体积分数59.66%、匀浆转数11 999.6 r/min、液料比7.96∶1 mL/g、提取次数3次。在所确定的工艺条件下，刺五加紫丁香苷得率0.0016%，纯度0.052%；异秦皮啶得率0.018%，纯度0.68%；刺五加苷E得率0.035%，纯度1.10%。