Abstract: A novel matrix was developed for fermentation to produce L-lactic acid by Rhizopus oryzae at shaker-level. Fungal mycelia were immobilized on the matrices to study the fermentation conditions and process stability. During the seed culture process，all the spores were adsorbed after only 5 h，biomass concentration was up to 6.8g/L after 24 h. Under optimized conditions using three matrices at 33 ℃ and 180 r/min with volume ratio of 20% by adding CaCO3 in time，L-lactic acid was obtained after 48 h fermentation with concentration of 50.00 g/L in 65.0% yield and the productivity was 1.0 g/(L·h). Compared with cell-free fermentation，the concentration of L-lactic acid，the yield and the productivity increased by 79%，250% and 150%，respectively，and the fermentation period decreased by 24 h. The process of immobilized fermentation showed good stability in seven repeated batches.

摘要： 采用一种新型载体支架来进行米根霉的固定化发酵研究。种子培养过程中，5 h后孢子已全部吸附到支架上，种子培养时间为24 h，生物量维持在6.8 g/L以上；发酵过程中，在温度为33 ℃，摇床转速为180 r/min，装液量为20%，支架数为3个，时间为48 h，发酵过程中及时补加CaCO3的条件下，L（+）-乳酸平均浓度可达50.00 g/L，转化率接近65.0%，产率可保持在1.0 g/（L·h）以上。与游离发酵相比，固定化发酵乳酸浓度、转化率、产率分别提高了79%、250%和150%，发酵周期提前了24 h，且能重复发酵7个批次。