Abstract: Lipase is an important industrial enzyme. This review summarizes the application of Pseudomonas lipases in resolution of chiral alcohols and esters which are important intermediates for medical and fine chemicals. Besides，mechanism for the enantioselective performance of Pseudomonas lipase by molecular modeling，and the method for enantioselective enhancement by directed evolution are discussed. Furthermore，due to the nature of organic solvent tolerance，Pseudomonas lipases can be used in non-aqueous biocatalysis. With the interdisciplinary development of biology and chemistry，Pseudomonas lipases will have great potential in development and application in the future.

摘要： 脂肪酶是一种重要的工业用酶，本文从酶源角度综述了假单胞菌脂肪酶在拆分手性醇和酯等精细化学品中的应用，通过分子模拟解释其选择性并通过定向进化提高其选择性。由于假单胞菌脂肪酶天然耐有机溶剂的特性，可用于非水相生物催化。随着生物和化学领域的不断交叉，将进一步推动假单胞菌脂肪酶的开发和应用。