Abstract: Enzyme must be treated properly for properties improvement before implementation at industrial scale，in which enzyme immobilization is the most important and conventional technique. This paper introduces the recent process in enzyme immobilization，including multipoint covalent immobilization，single enzyme nanoparticles，magnetic nanofibers，cross-linked enzyme crystal，cross-linked enzyme aggregate，chaperonin，artificial oil bodies and aptamer. New strategies to obtain immobilized enzyme with improved catalytic properties are emphasized，including stability，activity，enantioselectivity，recovery and reuse.

摘要： 酶在工业化利用之前，必须要经过适当的处理，目的是改善酶的性能。酶固定化技术是改善酶的催化性能最重要、常用的手段。本文介绍了酶固定化技术的最新研究成果（如多点共价结合、单酶纳米粒、磁性纳米纤维、交联酶结晶、交联酶聚集体、分子伴侣、人工油体、适配体等），重点描述了通过固定化来改善酶的催化性能，包括酶的稳定性、活力、立体选择性以及回收利用性能等。