Abstract: Fatty acid methyl esters with different carbon chain length were separated by urea adduct method for providing preservation film materials for agricultural products. Effects of urea amount，solvent usage，adduction time and temperature on the separation were investigated. According to the Box-Benhnken center-united experimental design principles，three factors of urea amount，95% ethanol consumption and temperature，and two indexes of the purities of C16 and C18 fatty acid methyl esters were selected，the separation conditions were optimized by Design expert 7.0.1 software and the corresponding regression model was established. The optimized conditions were found as：palm oil methyl ester 20 g，urea amount 35 g，5% ethanol 120 mL，inclusion temperature of 5 ℃，inclusion time 16 h，under which the C16 fatty acid methyl ester content in the phase of saturated fatty acid methyl ester reached to 78.5%，the C18 fatty acid methyl ester content in the phase of unsaturated fatty acid methyl ester reached to 93.1%，improved 36.4% and 40.8% respectively than the starting materials.

摘要： 采用尿素包合法分离棕榈油甲酯化物中不同碳链长度的脂肪酸甲酯，为农产品涂膜保鲜材料的开发提供原料。重点考察了尿素用量、溶剂用量、包合时间和包合温度对分离效果的影响，并以尿素用量、95%乙醇用量、包合温度为三因素，C16脂肪酸甲酯和C18脂肪酸甲酯的纯度为二指标，根据Box-Benhnken中心组合试验设计原理，利用Design expert7.0.1软件分析优化了分离的工艺条件并建立了回归模型。优化的最佳工艺条件如下：在棕榈油甲酯化物用量为20 g，尿素用量为35 g，95%乙醇用量为120 mL，包合温度为5 ℃，包合时间为16 h的条件下，饱和脂肪酸甲酯相中C16脂肪酸甲酯的含量达78.5%，不饱和脂肪酸甲酯相中C18脂肪酸甲酯的含量达93.1%，分别比原料提高36.4%和40.8%。