Abstract: Pentachlorophenol (PCP) is one of persistent organic pollutants, which is widely used as wood preservative, fungicide and herbicide. Because of its toxicity and widespread distribution, it has worldwide contaminated soil, surface water and underground water. The immobilization conditions for the PCP degradation bacteria NERCDT-A, NERCDT-B, NERCDT-C and NERCDT-D were investigated with the orthogonal method including immobilization material selection, pH of solution and amount of inoculation. The immobilized bacteria showed higher degradation efficiency for PCP than the bacteria in solution. The Monod parameters of different immobilized bacteria have been obtained from the degradation kinetics.

摘要： 五氯苯酚（PCP）是一种曾被广泛使用的木材防腐剂、杀菌剂和除草剂，目前已经造成了世界范围内土壤和水体的污染。本文针对筛选的4种优势菌株NERCDT-A、NERCDT-B、NERCDT-C、NERCDT-D，通过正交实验考察不同固定化材料、溶液pH值和生物接种量，确定出适宜的菌株固定化包埋条件。分别用游离单株优势菌和固定单株优势菌对PCP进行降解实验研究，对比结果表明固定化的菌株表现出了较好的降解效果。通过固定化菌株对PCP的降解动力学研究，确定不同固定化菌株的Monod参数。