Abstract: Exponential fed-batch culture strategy were carried out to produce cholesterol oxidase (COD) using recombinant E. coli high cell-density culture. High cell-density was achieved by specific growth rate grading-control，and the final cell dry weight was 40.128g/L. The optimum induction time was determined as in the mid-logarithmic phase. Under the optimized cultivation conditions，the COD production rate was 1287.31U/(L•h). The highest COD activity (6436.56U/L) and highest COD productivity [459.75U/(L•h)] were achieved.

摘要： 以指数流加策略高密度培养重组大肠杆菌，发酵生产胆固醇氧化酶。通过对比生长速率的分阶段控制使得细胞干重达40.128 g/L。确定了最佳的诱导时机为菌体对数生长期的中期，产酶速率为1287.31 U/(L•h)。菌体产酶水平达6436.56 U/L，生产强度为459.75 U/(L•h)，实现了胆固醇氧化酶的高效生产。