Chemical Industry and Engineering Progree

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Exponential fed-batch culture strategy for the production of cholesterol oxidase in recombinant E. coli JM109-COD

SUN Yan,WANG Changcheng,ZHANG Ling,YANG Hailin,WANG Wu   

  1. Key Laboratory of Industrial Biotechnology of Ministry of Education,Jiangnan University
  • Online:2010-01-05 Published:2010-01-05

重组大肠杆菌产胆固醇氧化酶的指数流加策略

孙 艳,王长城,张 玲,杨海麟,王 武   

  1. 江南大学工业生物技术教育部重点实验室

Abstract: Exponential fed-batch culture strategy were carried out to produce cholesterol oxidase (COD) using recombinant E. coli high cell-density culture. High cell-density was achieved by specific growth rate grading-control,and the final cell dry weight was 40.128g/L. The optimum induction time was determined as in the mid-logarithmic phase. Under the optimized cultivation conditions,the COD production rate was 1287.31U/(L•h). The highest COD activity (6436.56U/L) and highest COD productivity [459.75U/(L•h)] were achieved.

摘要: 以指数流加策略高密度培养重组大肠杆菌,发酵生产胆固醇氧化酶。通过对比生长速率的分阶段控制使得细胞干重达40.128 g/L。确定了最佳的诱导时机为菌体对数生长期的中期,产酶速率为1287.31 U/(L•h)。菌体产酶水平达6436.56 U/L,生产强度为459.75 U/(L•h),实现了胆固醇氧化酶的高效生产。

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