Abstract: The preparation conditions of alginate-chitosan-alginate (ACA) microcapsules with liquid core and good mechanical strengthwere optimized by response surface method. In the first optimization step，a Plackett-Burman design was used to evaluate the influence of related factors. The concentration and pH of chitosan solution have a positive effect to the membrane mechanical strength，while the pH of sodium citrate has a negativeeffect. The rest of other preparation conditions has no significant influence on the membrane mechanical strength. The path of steepest ascent was used to approach the optimal region of the optimized conditions subsequently. In the third step，the concentration and pH of chitosan solution and the pH of sodium citratethe were further optimized using central composite designs and response surface analysis. Verification experiment under the optimized conditions presented a Sw(%) value of －14.62%，which is in line with the theoretical value of －15.41%. Under the optimized condition，ACA microcapsules with liquid core demonstrate good performance in mass transferring and biocompatibility.

摘要： 采用响应面法优化工艺条件，制备具有较好机械强度的海藻酸盐-壳聚糖-海藻酸盐（ACA）液芯微胶囊。首先，通过Plackett-Burman方法对相关影响因素的效应进行评价，筛选出有显著效应的壳聚糖浓度、壳聚糖溶液pH值和柠檬酸钠溶液pH值3个因素；其次，采用最陡爬坡实验接近具有最好膜强度的中心点区域；最后由中心组合实验和响应面分析确定主要影响因子的最佳条件。在优化工艺条件下验证膜的机械强度，膨胀率－14.62%与优化结果－15.41%接近，优化结果与实际情况较好吻合。在优化条件基础上进行传质性能和细胞生长特性实验，研究结果表明ACA液芯微胶囊比ACA固芯微胶囊具有更好的传质特性，将ACA液芯微胶囊进行细胞包埋，液芯微胶囊较固芯微胶囊具有更高的细胞密度。