Chemical Industry and Engineering Progree

• 生物化工 • Previous Articles     Next Articles

Study on production of reducing sugars from bagasse by penicillium sp.L8

LUO Guangwen,LIAO Xiaoxiang,CHEN Mingqiang,LIANG Bing   

  1. Department of Pharmaceutical and Biological Engineering, Sichuan University
  • Online:2009-07-05 Published:2009-07-05

青霉菌L8降解蔗渣产还原糖的发酵

罗广文,廖晓祥,陈明强,梁 冰   

  1. 四川大学化工学院制药与生物工程系

Abstract: A mould was selected from rotten wood and was identified to be a penicillium sp. The strain’s conditions for enzyme production and enzyme-catalyzed temperature were tested.Then orthogonal test showed the basic fermentation conditions was:bagasse microwave-alkali pretreatment time 160s, no addition of tween80, inoculation amount 10%,culture time 5d.The fermentation conditions were further optimized by single factor tests. Saccharification rate reached 41.8% under the optimal condition.The reducing sugars contained in the fermentation broth were preliminary identified to be glucose and xylose by TLC detection. The research results suggest it is worth further researching in the utilization of bagasse resource.

摘要: 从朽木下土壤筛选到一株霉菌,该菌初步鉴定为青霉菌。考察了该菌产酶时间及酶促温度,然后正交实验表明基本发酵条件为:微波-碱预处理蔗渣时间160 s,不添加吐温80,接种量10%,培养时间5天。单因素实验进一步优化了发酵条件。最优条件下蔗渣糖化率最高可达到41.8%。降解液经TLC检测,初步确定所含还原糖为葡萄糖和木糖。研究表明,青霉L8菌株在蔗渣综合利用上值得进一步研究。

京ICP备12046843号-2;京公网安备 11010102001994号
Copyright © Chemical Industry and Engineering Progress, All Rights Reserved.
E-mail: hgjz@cip.com.cn
Powered by Beijing Magtech Co. Ltd