Abstract: The optimum immobilization condition and the performance of the immobilized beads in reducing Fe2+ (EDTA)-NO was investigated. The beads embedded for 16 h with 4 % sodium alginate，200 mg/L microorganisms and 2% calcium chloride had good performance in reduction ability. However，they had a low mechanical strength，and were easy to be ruptured and swelled. The mechanical properties of the immobilized beads were improved greatly by first adding 2% activated carbon and 4% diatomite into sodium alginate to embed the microorganisms，and then crosslinking the beads with 0.02mol/L hexanediamine for 60min. In comparison with free bacteria，the immobilized bacteria had a large improvement in reduction performance and resistance to high-temperature and acid.

摘要： 采用海藻酸钠包埋驯化得到的优势还原菌，用于还原BioDeNOx工艺中吸收NO后形成的Fe2+(EDTA)-NO。最佳包埋条件为：海藻酸钠质量浓度4%，菌体质量浓度200 mg/L，CaCl2质量浓度2%，包埋时间16ｈ。此条件下制备的小球还原性能好，但机械强度不高，溶胀破裂现象严重。在此基础上，加入2%活性炭和4%硅藻土改良小球，并将制备的小球进一步用浓度0.02 mol/L己二胺交联60 min，得到的小球综合性能良好。与游离微生物相比，其还原性能、耐高温性和耐酸性也有较大程度提高。