Abstract: Wax ester was synthesized from soybean oil and cetyl alcohol in a packed-bed reactor with petroleum ether as solvent and immobilized lipase Candida sp.99-125 as catalyst. The effects of the height of packed-bed，flow rate of reactant，dosage of immobilized lipase，molar ratio of substrate were investigated. The optimum conditions were as follows：immobilized lipase 47g，the height of packed bed was 40cm (diameter 3.5cm)，the molar ratio of oil to cetyl alcohol was 1∶2.4，the flow rate of reactant was 4mL/min and the retention time was 4h，the yield of wax ester reached 83% .The stability of the immobilized lipase was also studied，and the yield remained above 70% after 16 batches. The packed mode of the immobilized lipase in reactor was discussed.

摘要： 研究了填充床式反应器酶法合成生物蜡酯的生产工艺。以大豆油、十六醇为原料，在石油醚体系中，使用实验室自制的固定化Candida sp.99-125为催化剂。对操作参数，如填充高度、进料速度、固定化酶用量、底物摩尔比进行了研究。结果表明，固定化酶质量为47 g，填充高度40 cm（内径3.5 cm），油醇摩尔比1∶2.4，以4 mL/min速度进料保留时间4 h，产率可达83%。固定化酶在使用16批时，产率仍保持在70%以上。同时，对于填充床的填充方式作了初步探索。