Abstract: Glucose and ethanolamines were catalytic hydrogenated to obtain N-(2-hydroxyethyl)- glucamine，with a transformation rate of 89%. N-(2-hydroxyethyl)-glucamine was dehydrogenated to 6-(2-droxyethyl)amino-6-deoxy-a-L-sorbofiuanose the key intermediate of miglitol by the resting cells of Gluconobater oxydans Gouv2007 through aeration. 6-(2-Droxyethyl)amino-6-deoxy-a-L- sorbofiuanose was hydrogenated catalyticlly to miglitol. In the transformation of N-(2-hydroxyethyl)- glucamine to miglitol，the transformation rate was 77.3%. The samples of N-(2-hydroxyethyl)- glucamine and miglitol were characterized with infrared spectroscopy，melting point and MALDI-MS analysis. The results confirmed the samples of N-(2-hydroxyethyl)-glucamine and miglitol.

摘要： 以葡萄糖和乙醇胺为原料，催化加氢生成N-羟乙基葡糖胺，葡萄糖转化率为89%。氧化葡萄糖酸杆菌Gouv2007的静息细胞在通气下氧化N-羟乙基葡糖胺脱氢生成米格列醇的前体物质6-脱氧-6-羟乙基氨基-α-L-呋喃山梨糖，再催化加氢生成米格列醇，对从N-羟乙基葡糖胺生成米格列醇的底物转化率为77.3％。采用熔程测定、红外光谱分析、质谱分析对N-羟乙基葡糖胺和米格列醇进行了表征，结果表明：所合成的产物分别被鉴定为N-羟乙基葡糖胺和米格列醇。