Abstract: A series of new carriers with different degrees of crosslinking were synthesized by suspension polymerization with glycidyl methacrylate (GMA) as functional monomer，ethylene glycol dimethacrylate (EGDMA) and divinyl benzene (DVB) as cross-linking agents. Mercury porosi-metry was used to characterize the pore structure（average pore diameter and surface area reached 14.1 nm，112.286 m2/g，respectively）. The swelling behavior of carriers in different solvents was also studied. At the same time，the functionalization of carrier was investigated，and the results showed that the carriers could react with amino group readily. The weak base exchange capacity，which reached 7.72 mmol/g in isopropanol was discussed. Finally，immobilization reaction of papain indicated that the activity of enzyme carriers was the highest in polyethylene polyamine，at a value of 2144 U/g.

摘要： 通过悬浮聚合，以甲基丙烯酸缩水甘油酯（GMA）为功能单体，二甲基丙烯酸乙二醇酯（EGDMA）和二乙烯基苯（DVB）为交联剂，合成不同交联度的新型固定化酶载体。利用压汞仪分析测定了载体的孔结构（平均孔径14.1 nm、比表面积达到112.286 m2/g），研究了该新型载体在不同溶剂中的溶胀行为。通过对载体的功能化研究，发现载体分子链上的环氧基易于与胺基发生反应，并讨论了胺化树脂弱碱交换量，其在异丙醇中达到7.72 mmol/g。最后通过与木瓜蛋白酶的固定化反应表明以多乙烯多胺为胺化剂制得的酶载体活性最高，测得为2144 U/g。