Abstract: Tert-amyl alcohol was used as a reaction medium for Novozym 435 lipase-catalyzed methanolysis of soybean oil for biodiesel production. With tert-amyl alcohol as reaction medium，both the negative effects caused by excessive methanol and by-product glycerol could be eliminated. The highest biodiesel yield of 97% was obtained under the optimum conditions (tert-amyl alcohol/oil volume ratio 1，methanol/oil molar ratio 3，2% Novozym 435 based on the soybean oil weight，temperature 40 ℃，2% water content based on soybean oil，180 r/min and 15 h). Moreover，there was no evident loss in lipase activity and stability even after being repeatedly used for 160 runs and the yield of biodiesel production was more than 95%.

摘要： 叔戊醇作为反应介质，固定化脂肪酶Novozym 435催化大豆油与甲醇的转酯反应制备生物柴油。叔戊醇消除了反应底物甲醇及反应副产物甘油对酶活的负面影响。定量分析表明，叔戊醇与油脂的体积比为1，甲醇与油脂的摩尔比为3，2%脂肪酶，反应体系含水量2%，40 ℃、180 r/min条件下反应15 h，生物柴油得率可达97%。在最适条件下反应进行160批次，酶仍保持了较高的活性和良好的稳定性。