Abstract: An aerobic denitrifying bacterial strain was loaded on nano Fe3O4 and applied to deal with actual living wastewater. The effects of free bacterial strains and immobilized bacterial strains for living wastewater treatment were compared. The optimum treatment conditions of free bacterial strains for wastewater are pH=6.8，30℃and 2 days of incubation time.，while those of immobilized bacterial strains are pH=7.8，30℃ and 3∶1 of Fe3O4/ dry weight of bacteria. The treatment effects of the bacterial strains are evidently improved by the immobilization on nano Fe3O4. The removal rates of total nitrogen，inorganic phosphorus and COD attained to 75.56%，38.83% and 87.5%，respectively. The bacterial strains loaded on nano Fe3O4 maintained high activities to remove total nitrogen，inorganic phosphorus and COD even repeatedly regenerated，which have broad application prospects in the area of nutrition –rich wastewater treatment.

摘要： 以纳米Fe3O4负载好氧反硝化菌处理生活废水，确定了游离菌及固定化菌处理废水的最佳条件及处理效果。游离菌在pH=6.8、30℃条件下作用2天，对废水的处理效果最佳，而固定化菌在pH=7.8、30℃及Fe3O4/菌=3∶1时，对废水的处理效果最佳，菌株经纳米Fe3O4负载后，对废水的脱氮、除磷及去除COD效果得到明显提升，去除率分别达到75.56%、38.83%及87.5%。纳米Fe3O4负载的菌株经反复再生，并连续处理4批废水，总氮、无机磷及COD的去除效果仍然维持在高水平，在治理水体富营养化领域具有广阔的应用前景。