Abstract: With the necessary carbon source for cell culture， glucose and citric acid and the nitrogen source- L-glutamic acid as the small molecul models，and a series of poly(ethylene-glycol)s (PEG) of different molecular weights as the large molecule models，the permeability of chitosan/alginate microcapsules was studied and the influence of preparation conditions on microcapsules permeability was investigated. Experimental results showed that glucose，L-glutamic acid，citric acid could diffuse through the membrane into the microcapsules，the molecular mass cut-off (MMCO) of microcapsules prepared from 20 mg/mL sodium alginate and 2 mg/mL chitosan of molecular weight 50 000 was approximately 1500，and the peameability of chitosan/alginate microcapsules increased with the decrease of the concentration of chitosan solution and chitosan molecular weight，and the increase of microcapsule diameter.

摘要： 以传统细胞培养的重要碳源葡萄糖与柠檬酸、氮源L-谷氨酸为小分子模型，以聚乙二醇为测定微囊膜截留相对分子质量的模型分子，研究了微胶囊通透性能，考察了制备条件对壳聚糖/海藻酸钙微胶囊通透性能的影响。结果说明：葡萄糖、柠檬酸和L-谷氨酸可以自由透过微囊膜扩散；采用20 mg/mL海藻酸钠溶液与相对分子质量5万、2 mg/mL壳聚糖溶液制备微胶囊，胶囊的截留相对分子质量为1500；降低成膜壳聚糖溶液浓度和壳聚糖分子量，增大微胶囊粒径均有利于提高壳聚糖/海藻酸钙微胶囊的通透性。