Abstract: Enhancement of asymmetric reduction efficiency for (R)-mandelic acid (MA) from phenylglyoxylic acid (PGA) was investigated with whole cells of Saccharomyces ellipsoideus as catalyst. Utilization of fed-batch and addition of adsorbent resin could be effective in increasing substrate concentration and mitigating substrate inhibition. Absorbent resin NKAⅡ was selected，which had a large adsorption amount and a high adsorption rate of the substrate. By adding NKAⅡ adsorption resin in the reaction system，substrate inhibition was mitigated，and the initial substrate concentration was thus increased. Finally it was found that combining these two methods was the most effective way. Substrate concentration was increased from 33.31 mmol/L to 166.53 mmol/L, and a 95.6% yield of (R)-mandelic was obtained with fed-batch in a resin-added system. Reusability of cell was satisfactory.

摘要： 以葡萄酒酵母Saccharomyces ellipsoideus为催化剂，对提高不对称还原苯乙酮酸生产(R)-扁桃酸效率进行了研究，分别利用了分批补料、添加吸附树脂的方法降低底物对反应过程的抑制作用，提高反应的底物浓度。并且通过对吸附树脂吸附性能的考察，筛选出了一种对底物有较快吸附速率和较大吸附量的树脂NKAⅡ，可以显著降低底物对反应过程的抑制，提高反应的底物浓度。最终发现将两种方法结合，在添加吸附树脂的体系中进行分批补料是最高效的方法，将底物浓度从33.31 mmol/L提高到166.53 mmol/L，并且产率达到了91.6%，e.e.值为100%，而且菌体重复使用性能很好。