Abstract: By using precipitation polymerization，thermo-sensitive poly(N-isopropylacrylamide-co- glycidyl methacrylate) [P(NIPAM-co-GMA)] microspheres were synthesized via the one-step and two-step reaction methods. The one-step method proceeded with the polymerization reaction of all reactants at the same time，while the two-step method proceeded by the polymerization reaction of NIPAM firstly，and then adding GMA to form P(NIPAM-co-GMA) microspheres. The poly(N- isopropylacrylamide-co-2- hydroxypropyl methacrylate/ethylenediamino-β-cyclodextrin) [P(NIPAM-co- GMA/β-CD)] microspheres were fabricated through chemical reaction of P(NIPAM-co-GMA) microspheres with modified ethylenediamino-β- cyclodextrin (EDA-β-CD). The morphologies，chemical structures and thermo-sensitive properties of all prepared microspheres were characterized by scanning electron microscopy，Fourier transform infrared spectroscopy，dynamic light scattering and microscope. The results showed that the prepared microspheres by both methods had good monodispersity and sphericity. The microspheres with β-CD groups were prepared successfully，and all microspheres had good thermo-sensitive properties. In addition，microspheres prepared by the two-step method were larger and hadve more pendent β-CD groups than that prepared by the one-step method.

摘要： 采用沉淀聚合机理，由一步法和两步法制备聚（N-异丙基丙烯酰胺-甲基丙烯酸缩水甘油酯） [P(NIPAM-co-GMA)]温敏性微球。其中一步法是同时加入所有反应物反应成微球，而两步法是先加NIPAM成微球，再加入GMA，最终均生成P(NIPAM-co-GMA)微球；再将改性的乙二胺代环糊精（EDA-β-CD）通过化学反应引入到P(NIPAM-co-GMA)微球结构中，制备得到聚（N-异丙基丙烯酰胺-甲基丙烯酸-2-羟丙基乙二胺基环糊精） [P(NIPAM-co-GMA/β-CD)]共聚高分子微球。分别用扫描电镜、红外光谱、控温激光粒度仪及光学显微镜对产物的形貌、结构和温敏特性进行了表征。结果表明，两种方法制备的微球均具有良好的单分散性和球形度，均能成功地固载β-环糊精（β-CD）基团，并且都有温度响应特性；但是，同一步法制备的微球相比，两步法制得的微球粒径明显较大，且微球固载有更多的β-CD。