Abstract: The determination of residual pesticides is mainly divided into four processes：extraction，cleanup，concentration and detection. The methods for extracting samples include shaking extraction，soxhlet’s extraction，solid phase micro-extraction，supercritical fluid extraction，accelerated solvent extraction and so on. The cleanup methods include liquid-liquid partition process，column chromatography，sulfonation and so on. The concentration methods include evaporation and concentration，reverse-osmosis，Kuderna Danish (K-D) concentrator and so on. The detection methods include gas chromatography-mass spectrometry，fluorometry，enzyme inhibition，immunoassay，biosensor and infrared spectrometry，Raman spectrometry，and so on. The advantages and disadvantages of these methods were reviewed and the development tendency in the future was pointed out.

摘要： 介绍了农药残留检测的步骤与方法。农药残留检测主要分为4个步骤：样品的提取、净化、浓缩和检测。提取方法主要有震荡法、索式提取法、固相微萃取法、超临界流体萃取法、快速溶剂萃取法等。净化方法主要有：液-液分配净化法、柱层析法、磺化法等。浓缩方法主要有：蒸发浓缩、反渗透浓缩、K-D浓缩仪浓缩等。检测方法主要有：气相色谱-质谱联用法、荧光分析法、酶抑制法、免疫分析法、生物传感器检测法、红外光谱法、拉曼光谱法等。评述了这些方法的优点与缺陷，提出了今后的发展方向。