Abstract: Resin DA201 was screened out from several adsorbent resins. The resin had less adsorption ability for enzyme and coenzyme (adsorption rates 13.13% and 14.5% respectively) with no damge to enzyme activity，but at the same time，had strong adsorption ability for 1,3-PD and DHA (adsorption rates 23% and 27% respectively); The resin was used to adsorb and separate 1,3-PD and DHA，recycle enzyme and coenzyme to continue 1,3-PD enzyme catalyse with NADH regeneration; The conditon for the resin to have the preferable adsorption effect was mass of resin 4.8 times greater than 1g/L standard substrate，adsorption time 6 h. The 1,3-PD was continuously prepared by two batchs under this condition; 1,3-PD conversion rate was 42.9% and 35.5% for the first and second batches respectively，and total conversion rate was 39.72%; The result showed that the adsorption resin had certain separation effect for substrate，product，enzyme and coenzyme，and could effectively realize continuous enzyme catalyse reaction with coenzyme regeneration.

摘要： 从几种吸附树脂中筛选出了树脂DA201。树脂DA201对酶、辅酶吸附较少（吸附率分别为13.13%，14.5%）且对酶活无较大破坏，对产物1,3-丙二醇（1,3-PD）与二羟基丙酮（DHA）吸附较多（吸附率分别为23%，27%）。利用树脂DA201进行产物的富集分离及酶、辅酶回流再利用，实现伴有辅酶NADH再生的1,3-PD酶法连续反应。在底物浓度为1 g/L时，树脂质量浓度为底物浓度的4.8倍、吸附时间为6 h，树脂DA201对底产物有较好的吸附分离效果。在此条件下进行1,3-PD的2个批次连续制备，第1、第2批次1,3-PD转化率分别为42.9%、35.5%，总转化率为39.72%。实验结果说明，吸附树脂对底产物、酶与辅酶具有一定的分离作用，利用树脂的分离作用可有效地实现伴有辅酶再生的酶催化反应的连续进行。