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Isolation and degradation characteristics of m-cresol degrading strains with high efficiency

FU Liu,REN Yuan,WEI Chaohai   

  1. College of Environmental Science and Engineering,South China University of Technology
  • Online:2008-07-05 Published:2008-07-05

间甲酚高效降解菌的筛选及其降解特性

付 柳,任 源,韦朝海   

  1. 华南理工大学环境科学与工程学院

Abstract: The m-cresol degrading composite strains were isolated from the aerobic sludge of coking wastewater of Guangdong SGIS Co. Ltd.,and were named CDMs (cresol degrading micro-organisms) according to their function. In shaking flasks experiments,the effects of degradation conditions were studied and the optimum pH,inoculums amount,temperature and concentration of metal ions usually present in wastewater were determined. At pH 6.2~7.2,inoculums amount 10%~15% and temperature 35~40 ℃,the degradation of m-cresol was efficient. The presence of K+,Na+,Ca2+,Fe3+ and Mg2+ ions could improve the biodegradation ability when they were 50 mg/L,2000 mg/L,3 mg/L,1.5 mg/L and 4~10 mg/L respectively while the presence of Cu2+ ion could restrain the reaction seriously. Under specified conditions,the degradation with different initial concentrations exhibited first order kinetics characteristics. When the initial concentration was 551 mg/L,the average degradation rate reached a maximum,8.4 mg/ (L·h),and the composite strains were the most efficient and toxicity-resistant for m-cresol degradation.

摘要: 以广东韶钢焦化厂废水处理站好氧池中的污泥作为菌源,分离出能降解间甲酚的复合菌种,根据其功能性命名为CDMs(cresol degrading micro-organisms)。通过摇瓶试验考察了环境条件对该菌种降解间甲酚的影响,确定了最适pH值、接种量、温度及常见金属离子浓度影响的范围。得到间甲酚的最适降解条件为pH值6.2~7.2、接种量10%~15%、温度35~40 ℃。当K+、Na+、Ca2+、Fe3+和Mg2+的浓度分别为50 mg/L、2000 mg/L、3 mg/L、1.5 mg/L及4~10 mg/L时,CDMs对间甲酚的降解表现出高活性。Cu2+严重抑制该降解过程。在一定环境条件下,CDMs对不同初始浓度间甲酚的降解符合一级反应动力学特征。当间甲酚初始浓度为551 mg/L时的平均降解速率达到8.4 mg/(L· h)。表明所分离得到的CDMs在适宜条件下可高效降解间甲酚并体现出较强的毒性耐受能力。

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