Abstract: Polystyrene (PS) membranes with pits of different sizes were presented by liquid phase separation，and then convex chitosan membranes were prepared with the PS membranes as templates. The surface morphology and structure were characterized by using scanning electron microscope，atomic force microscope，and the formation mechanism of membrane with pore structure was proposed. By changing the parameters，such as the volume ratio of glycerol (Gly) and propylene glycol (PG)，the temperature of film formation and the rate of evaporation，the membranes with different surface morphologies and microstructures could be obtained. The PS films patterned with concavities were used as templates to fabricate convex patterned chitosan membranes. The sizes of the convexes were determined as about 1～50 μm by scanning electron microscope and atomic force microscope. And the air-water contact angles decreased with the increase of convex size. It could be the biomaterial basis for studying the biological function of cells with different surface convexes.

摘要： 用液致相分离法制备多孔聚苯乙烯膜，探讨多孔相变膜的成膜机理和孔隙结构形成的原因，并以此为模板制备不同表面凸起的壳聚糖膜，对膜的形态结构进行扫描电镜和原子力显微镜观察。结果表明，调节丙三醇和1,2-丙二醇体积比、成膜温度、挥发速度可制备出不同表面形态和具有1～50 μm不同微观孔洞的PS膜。以PS膜为模板，制备不同表面凸起的壳聚糖膜，扫描电镜和原子力显微镜测定了凸起的大小和高度，其水相接触角随着凸起大小的减小而减小，亲水性亦减小。这为进一步评价壳聚糖膜表面微观结构对细胞黏附、生长的影响奠定了材料学基础。