Abstract: High speed counter-current chromatography (HSCC) was applied to the separation of anti-tumor components from an unidentified endophytic fungi. A biphasic solvent system composed of n-hexane-ethyl acetate-methanol-water (1∶3∶3∶3，volume ratio) was used. The bioactive compound purified and collected was analyzed by high-performance liquid chromatography. A white powder with purity above 99% was obtained. The chemical structure of the bioactive compound was identified with ESI-MS，1H NMR and 13C NMR as cytochalasin N.

摘要： 利用高速逆流色谱法对一株内生真菌活性代谢产物进行了分离。两相溶剂系统为正己烷-乙酸乙酯-甲醇-水（体积比为1∶3∶3∶3），固定相为系统上相，下相为流动相。从内生真菌的代谢物中分离得到一抗肿瘤细胞活性组分。经高效液相色谱分析，其纯度大于99%以上。其化学结构由ESI-MS、1H NMR 和13C NMR确认为Cytochalasin N。