Abstract: 1,3-propanediol (1,3-PD) is an important intermediate in the production of polyesters propylene terephthalate (PTT). Compared with the chemical method，the production by microbial fermentation has increasingly attracted more attention for its friendliness with the environment. In this paper，the research advances in genetic modification of Klebsiella pneumoniae are reviewed with regard to metabolic pathway，expression host，vector construction，and key enzymes in the conversion of glycerol to 1,3-propanediol. Moreover，some prevailing protocols for strain selection and genetic modification are also discussed.

摘要： 1,3-丙二醇是合成聚对苯二甲酸丙二醇酯（PTT）的重要原料。与化学法相比，微生物发酵生产1,3-丙二醇因对环境友好而成为当今研究热点。本文作者以肺炎克雷伯菌（Klebsiella pneumoniae）为表达宿主，从代谢途径、表达宿主、载体构建和关键酶基因等方面，综述了发酵菌种遗传改造的研究进展，并对一些新的菌种筛选和改造方法进行了讨论。