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Characteristics of a mixed culture for degradation of poly(vinyl alcohol) and optimization of culture conditions

NIU Haiyan,DU Guocheng,CHEN Jian   

  1. Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University
  • Online:2008-02-05 Published:2008-02-05

一个降解聚乙烯醇混合菌系的降解特性及培养基
碳氮源优化

牛海燕,堵国成,陈 坚   

  1. 江南大学生物工程学院工业生物技术教育部重点实验室

Abstract: A mixed culture,which could degrade 1 g/L poly (vinyl alcohol) (PVA) completely,was obtained from a soil sample from one Textile Factory. The effect of different concentrations of carbon and nitrogen sources was investigated in the mixed system. The results indicated that organic nitrogen sources were more suitable for this mixed culture to degrade PVA than inorganic nitrogen sources. Three strains were isolated from the PVA-degrading microbial population through the dilution method. Among them two belonged to bacteria and one was mould. One of the bacteria and the mould could produce extracellular PVA-degrading enzyme,but none of them could degrade PVA completely alone.

摘要: 从某纺织厂筛选到一个能够降解1 g/L PVA的混合菌系,研究了碳、氮源对该混合菌系降解PVA的影响。实验表明,有机氮源比无机氮源更适合该混合体系降解PVA。通过平板稀释涂布从该混合菌系中分离出3株可以利用PVA的菌株,其中2株细菌和1株霉菌。其中1株细菌和霉菌能产生胞外PVA降解酶,但它们产生的胞外酶只能部分降解PVA。

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