Abstract: Bioleaching of chalcopyrite with mesophile Thiobacillus ferrooxidans was performed at a low temperature (8～10℃). The results showed that the leaching rate of chalcopyrite for copper in sulfuric acid medium in 90 days was only 16.08%，the leaching rate in ferric sulfate medium was 21.3%，but for bioleaching it was as high as 37.96%. The optimal conditions for chalcopyrite bioleaching with Thiobacillus ferrooxidans were pH 2.0，10% inoculation，100 mesh ore size，2% slurry concentration. At the initial stage of the bioleaching process，adding 6g/L Fe2+ would greatly enhance bioleaching rate for copper to 53.51%.

摘要： 使用中温菌氧化亚铁硫杆菌（Thiobacillus ferrooxidans）在低温条件（8～10 ℃）下浸出黄铜矿。结果表明：经过90d的低温摇瓶浸出后，在无菌硫酸浸出过程中，黄铜矿可以自发氧化分解，最终浸出率为16.08%；在无菌高铁硫酸浸出过程中，由于Fe3+起到了一定的氧化作用，最终浸出率为21.3%；在有菌浸出过程中，氧化亚铁硫杆菌在低温下的浸出率可达37.96%。氧化亚铁硫杆菌在低温下浸出黄铜矿的最适pH值为2.0，在接种量为10%，矿石粒径为0.16 mm，矿浆浓度为2%时较为适宜。在低温浸出的初始阶段，当添加Fe2+的量为6 g/L时能促进浸出率提高最大，最终可达到53.51%。