Abstract: Enzymatic synthesis of wax ester was studied by using rapeseed oil and cetyl alcohol as substrate for the immobilized lipase Candida sp. 99-125 in the laboratory in a organic solvent system and a solvent-free system separately. Several factors which influencing wax ester synthesis were investigated. The reaction could proceed in the presence of hexane and the highest yield of wax ester （85.87％） was obtained with reaction temperature of 40℃，reaction time of 12hr，substrate molar ratio （oil to alcohol）of 1∶2，enzyme dosage of 15% and water content of 7.5%. The research on reaction conditions also showed that the yield of wax ester could reach 80.68 % in a solvent-free system when reaction temperature was 50℃，reaction time was 20hr and substrate molar ratio was 1∶1.5. The life span of the immobilized lipase could be higher by adding cetyl alcohol.

摘要： 以菜籽油、鲸蜡醇为原料，采用实验室自制的固定化Candidia sp.99-125脂肪酶分别在有机溶剂和无溶剂体系下合成了蜡酯，在两种体系下对影响蜡酯合成的各种因素进行了比较研究，研究表明：以正己烷为反应介质，在40 ℃条件下反应12 h，油醇摩尔比1∶2，酶量15%（质量分数，上同），水含量7.5%，蜡酯产率可达85.87%；无溶剂体系中在50 ℃条件下反应20 h，油醇摩尔比1∶1.5，蜡酯产率可达80.68%。通过流加鲸蜡醇可以提高酶的使用寿命。