Abstract: The effect of different types of surfactants on cell growth and hyaluronic acid (HA) production by Streptococcus zooepidemicus H24 was investigated. Among three surfactants, CTAB played a key role in cell growth and HA production. Compared with the control (without CATB), when 20 mg/L CTAB was added to the flask culture, the concentration of HA increased by 20%. Furthermore, the effect of cationic surfactant CTAB on cell growth and HA production was also investigated in a 2.5 L fermentor. The concentration of HA increased from 5.1 g/L to 5.7 g/L by adding CTAB during 8 h and 12 h. Those results indicated that CTAB enhanced HA production, and CTAB addition benefited the capsule removal of Streptococcus zooepidemicus during fermentation.

摘要： 研究了不同类型表面活性剂对兽疫链球菌Streptococcus zooepidemicus H24菌体生长和透明质酸发酵的影响，在此基础上，进一步研究了2.5 L发酵罐中阳离子型表面活性剂十六烷基三甲基溴化铵（CTAB）的添加对透明质酸发酵的影响。研究结果表明：摇瓶发酵时，发酵中后期（12 h）添加离子型表面活性剂有利于透明质酸产量的提高，在摇瓶中添加20 mg/L阳离子型表面活性剂CTAB使HA比对照组提高20%；发酵罐上8～12 h流加CTAB可使兽疫链球菌细胞荚膜脱落，促使透明质酸的产量由5.1 g/L提高到5.7 g/L，增加11.8%。本研究结果为进一步提高发酵法生产其他黏多糖的产量提供了一定的借鉴。