Abstract: β-glucanase produced by immobilized Escherichia coli JM 109-pLF3 in a packed bed bioreactor was studied. In batch fermentation，the highest enzyme activity of 100.3 U/mL was achieved at a recycling rate of 44.49 mL/min and an aeration rate of 0.6 mL/min after 48 h cultivation. In repeated batch operation，the enzyme activity up to 100 U/mL was obtained in 5 cycles，showing high stability of the immobilized cells. In continuous fermentation，the production of β-glucanase by immobilized cells was kept more or less constant. When the dilution rate was 0.05 h-1，the enzyme activity in the fermentation broth was 39.1 U/mL.

摘要： 以多孔陶瓷为载体吸附法固定重组大肠杆菌E. coli JM 109-pLF3表达胞外β-葡聚糖酶。考察了固定床间歇培养时循环流速和曝气量对发酵液酶活力的影响。当循环流速达到44.19 mL/min，曝气量达到0.6 mL/min时，培养48 h后，发酵液的酶活力达100.3 U/mL。固定化细胞具有良好的重复使用能力，在连续5批次实验中，培养48 h后的酶活力均在100 U/mL左右。固定床连续培养时，固定化细胞能够保持恒定的产酶效率，当稀释率为0.05 h－1时，发酵液中得到的酶活力为39.1 U/mL。