Abstract: The liquid chromatography-mass spectrometric (LC-UV/MS) and standard chromatographic fingerprints of macamides in maca were established. Combined with chemometrics technology,the quality control of maca and maca-based products had been achieved using comparative analysis of components. Different regions of dried maca hypocotyls and dietary supplement tablets were used as samples,and the macamides were extracted by oscillations with petroleum ether. The extracts were analyzed using LC-MS,and the results indicated that a better separation of commom peaks was obtained in 40mins and nine of these peaks were identified as characteristic macamide peaks by mass data. The overall similarity among eight kinds of maca dried hypocotyls and dietary supplements was no less than 0.76,which indicated that varieties of macamides in all samples were similar. Principal component analysis was performed using the data of whole spectrum and characteristic peaks of macamides as input variables,respectively. The results indicated that using the data of characteristic peaks as input variables was the better choice for quality control of maca. All samples were divided into three categories on the basis of their real origins and types of samples; and three of nine characteristic peaks had greater effect on the quality control. This method has some merits such as stable,efficient and accurate,and it is suitable for the quality control and the origin identification of maca.

Key words: maca, macamide, fingerprint, chemometrics, quality control

摘要： 建立玛咖中玛咖酰胺类化合物液相色谱-质谱联用（LC-UV/MS）标准指纹谱图，结合化学计量学技术对玛咖及其衍生产品进行组分比对分析，进而实现产品的质量控制。以不同产地的玛咖干根和保健品作为供试样品，采用石油醚进行震荡提取，提取液使用LC-UV/MS进行分析。共有峰在40min内得到了良好的分离，其中9个共有峰通过MS数据确证为玛咖酰胺指纹特征峰。对8种玛咖干根和保健品进行了相似度分析，整体相似度在0.76以上，说明供试样品的玛咖酰胺种类相似。分别以全谱和玛咖酰胺特征峰数据作为输入变量进行主成分分析，结果表明特征峰数据更适合作为质量控制的依据，样品可根据其真实产地和种类归为三类，其中3个特征峰对质量控制影响较大。该方法具有稳定、高效和准确的优点，可用于玛咖的质量控制和产地识别。

关键词: 玛咖, 玛咖酰胺, 指纹图谱, 化学计量学, 质量控制