Abstract: The adsorption and desorption rates of flavonoids in propolis by seven macroporous resins and polyamide were investigated，and it was found that AB-8 resin exhibited high adsorption and desorption capability which was suitable for enrichment of flavonoids. The factors (adsorption time and temperature，desorption temperature，ethanol eluent concentration，eluent volume) which influenced the enrichment of flavonoids in propolis were also studied，and a process to enrich total flavonoids in propolis was obtained. Adsorption at 35℃ water bath for 5h，when using 7BV 75% ethanol as eluent，desorption rate would reach up to 90%，and the total flavonoids purity of propolis extracts reached 30%.