化工进展

• 材料科学与技术 • 上一篇    下一篇

Fe3O4/P(AA-MMA-GMA) 磁性复合微球的制备及其

郭飞鸽,张秋禹,张和鹏,张宝亮   

  1. 西北工业大学理学院应用化学系,陕西 西安 710129
  • 出版日期:2010-09-05 发布日期:2010-09-05

Fe3O4/P(AA-MMA-GMA)magnetic composite microspheres,

GUO Feige,ZHANG Qiuyu,ZHANG Hepeng,ZHANG Baoliang   

  1. Chemistry Department,School of Science,Northwestern Polytechnical University,Xi’an 710129,Shaanxi,China
  • Online:2010-09-05 Published:2010-09-05

摘要:

采用新型的无皂乳液聚合法制备磁性复合微球,即以丙烯酸(AA)、甲基丙烯酸甲酯(MMA)和甲基丙烯酸缩水甘油酯(GMA)为单体,1,1-二苯基乙烯为自由基控制剂,无皂乳液聚合制备了一种磁性复合微球Fe3O4/PAA-MMA-GMA)。以所制备的Fe3O4/PAA-MMA-GMA)微球为载体,通过共价偶联山羊抗兔IgG抗体,得到了一种免疫磁性复合微球。研究表明,Fe3O4/PAA-MMA-GMA)微球表面带有环氧基,粒径为626 nm,具有超顺磁性。0.5 mg Fe3O4/PAA-MMA-GMA)微球与200 μg山羊抗兔IgG抗体在pH=7.4的磷酸盐缓冲液中于25 ℃反应16 h,可得到一种免疫磁性复合微球,其山羊抗兔IgG的偶联量为124 μg,且该免疫磁性复合微球可特异性结合兔抗肌动蛋白α

Abstract:

Magnetic composite microspheres of Fe3O4/PAA-MMA-GMAwere prepared via emulsifier-free emulsion polymerization of acrylic acidAAmethyl methacrylateMMAand glycidyl methacrylateGMAusing 1,1-diphenylethlene as free radical control agent. The obtained Fe3O4/PAA-MMA-GMAmicrospheres were then used to prepare immunomagnetic microspheres via the reaction with an antibody of goat anti-rabbit IgG. The Fe3O4/PAA-MMA-GMAmicrospheres showed superparamagnetism behavior with particle size of 626 nm bearing epoxy groups on the surface. The immunomagnetic microspheres were obtained by reacting 0.5 mg of Fe3O4/PAA-MMA-GMAwith 200 μg of goat anti-rabbit IgG in a phosphate buffer solution with pH 7.4 at 25 for 16 hthe coupling quantity for goat anti-rabbit IgG was 124 μg. The immunomagnetic microspheres can be specifically bound to rabbit anti-actin alpha.

京ICP备12046843号-2;京公网安备 11010102001994号
版权所有 © 《化工进展》编辑部
地址:北京市东城区青年湖南街13号 邮编:100011
电子信箱:hgjz@cip.com.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn