关键词: 安丝菌素P-3, 紫外诱变, 紫外-分光光度法, 培养基优化, 正交设计

Abstract: To improve the microbial fermentation yield of ansamitocinP-3 (AP-3), 96-well plate-microplate high-throughput screening method was established by UV-spectrophotometry. UV mutation breeding was carried out using the original strain (Actinosynnema pretiosum ssp. auranticum). A mutant strain B24-13 was obtained, and the higher production of AP-3 was 2.03-fold (reaching 112.5mg/L) compared with that original strain after 7 days fermentation, and the fermentation medium was optimized by culture medium optimization. After the analysis of the experimental results, the optimal medium for the mutant strain B24-13 was sucrose 25g/L, glycerol 1.5g/L, corn steep liquor 25g/L, CaCO₃ 7g/L, isobutanol 2g/L, valine 0.5g/L, MgSO₄ ·7H₂O, 0.5g/L, and FeSO₄·7H₂O 0.01g/L. Under those conditions, the yield of AP-3 was (127.5±6.3)mg/L after 7 days fermentation. The yield of AP-3 can be further improved by the mutagenesis breeding and the optimization of the culture medium. It is proved that the UV-spectrophotometry method is feasible for the rapid screening AP-3 high production strain.

Key words: ansamitocinP-3, UV mutagenesis, UV spectrophotometry, culture medium optimization, orthogonal design