碳氮源及pH调控对假肠膜明串珠菌产甘露醇的影响

doi:10.16085/j.issn.1000-6613.2015.12.033

化工进展 ›› 2015, Vol. 34 ›› Issue (12): 4333-4337.DOI: 10.16085/j.issn.1000-6613.2015.12.033

碳氮源及pH调控对假肠膜明串珠菌产甘露醇的影响

朱婧, 吴昊, 任心怡, 张敏, 马江锋, 姜岷

南京工业大学生物与制药工程学院, 材料化学工程国家重点实验室, 江苏南京 211816

收稿日期:2015-03-13 修回日期:2015-04-25 出版日期:2015-12-05 发布日期:2015-12-05
通讯作者: 姜岷,教授。E-mailbioengine@njtech.edu.cn。
作者简介:朱婧(1990—),女,硕士研究生。
基金资助:
江苏高校优势学科建设工程项目(PAPD)及新世纪优秀人才支持计划(NCET-12-0732)项目。

Effect of carbon and nitrogen source and pH control strategy on mannitol production by Leuconostoc pseudomesenteroides G123

ZHU Jing, WU Hao, REN Xinyi, ZHANG Min, MA Jiangfeng, JIANG Min

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China

Received:2015-03-13 Revised:2015-04-25 Online:2015-12-05 Published:2015-12-05

摘要/Abstract

摘要： 为了降低生物法制备甘露醇的成本,以假肠膜明串珠菌Leuconostoc pseudomesenteroides G123为研究对象,对培养基中的氮源、葡萄糖与果糖比例和pH调控过程进行了优化,提高了果糖转化率和甘露醇产量。5L发酵罐中结果显示:采用2g/L的酵母粉作为单一氮源,葡萄糖和果糖的比例为0.35:1,初始pH值7.5,发酵过程中保持pH值不低于4.5,甘露醇产量可达57.24g/L,甘露醇对果糖的转化率为83.2%。该过程副产D-乳酸20.32g/L,其光学纯度达99.9%,具有回收价值,甘露醇与D-乳酸对糖总转化率为89.38%,有助于降低生物法制备甘露醇的成本。

关键词: 甘露醇, 氮源, pH调控, D-乳酸

Abstract: A new mutant strain Leuconostoc pseudomesenteroides G123 was investigated in this study. The nitrogen source,the ratio of glucose and fructose and the pH control strategy were optimized to reduce the cost of mannitol production. Batch fermentations were operated in a 5L fermentor.The results showed that 57.24g/L mannitol was produced with the mannitol yield of 83.2% when 2g/L yeast extract was chosen as the single nitrogen source,the ratio of fructose toglucose was 0.35:1,and the initial pH was controlled at 7.5 and kept constant at 4.5 in the later phase. Besides,20.32g/L D-lactic acid with optical purity of 99.9% was also produced which suggested it could be recovered as a by-product. The yield of mannitol and lactic acid to the total sugar was 89.38%. As a result,the cost was reduced efficiently.

Key words: mannitol, nitrogen source, pH control strategy, D-lactic acid

中图分类号:

TQ923

朱婧, 吴昊, 任心怡, 张敏, 马江锋, 姜岷. 碳氮源及pH调控对假肠膜明串珠菌产甘露醇的影响[J]. 化工进展, 2015, 34(12): 4333-4337.

ZHU Jing, WU Hao, REN Xinyi, ZHANG Min, MA Jiangfeng, JIANG Min. Effect of carbon and nitrogen source and pH control strategy on mannitol production by Leuconostoc pseudomesenteroides G123[J]. Chemical Industry and Engineering Progree, 2015, 34(12): 4333-4337.

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碳氮源及pH调控对假肠膜明串珠菌产甘露醇的影响

Effect of carbon and nitrogen source and pH control strategy on mannitol production by Leuconostoc pseudomesenteroides G123

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