Abstract: Catalysts with different Ni content supported on activated alumina， γ-Al2O3 with surface area of 241 m2/g，were prepared via traditional volume impregnation method. The catalysts Ni/γ-Al2O3 were characterized by XRD，SEM and BET. Dehydrogenation of methylcyclohexane (MCH) catalyzed by Ni/γ-Al2O3 was performed under a fixed-bed micro reactor. Experimental results showed that the dehydrogenation conversion of MCH can be reached to 94.58% and the selectivity to toluene formation is 100% by using 20% Ni/γ-Al2O3 catalyst at 653 K and 0.5 MPa with v(MCH)/v(N2) of 7/12 and the volume gas space velocity of 212 h－1. These Ni-based catalysts are feasible to replace platinum and other noble metal catalysts.