关键词: 黏红酵母, 柠檬醛选择性加氢, 香叶醇

Abstract: Biological conversion of citral to geraniol was studied using Rhodotorula mucilaginosa CCZU-G5 as fermentation strain. The effects of culture time，co-substrate concentration，initial substrate concentration，cell quantity and the reaction time on selective hydrogenation of citral to geraniol were investigated to determine the optimal fermentation conditions. The results showed that after 28 h culturing，yeast cells reached the end of the logarithmic growth phase，in which the quantity and activity of the cells were at a high level. In the process of biological conversion，the optimal fermentation conditions were as follows：glucose concentration 40 g/L，initial citral concentration 4 g/L，yeast cell concentration 0.4 g/mL and reaction time 22 h. Under these conditions，citral conversion and geraniol selectivity reached 88.6% and 95.2%，respectively. The study lays the foundation for commercialized biosynthesis of geraniol.

Key words: Rhodotorula mucilaginosa, selective hydrogenation of citral, geraniol