固定化HRP/H2O2/ACAC酶促体系引发下降解淀粉接枝丙烯酰胺共聚物的合成与表征

化工进展

固定化HRP/H2O2/ACAC酶促体系引发下降解淀粉接枝丙烯酰胺共聚物的合成与表征

闫小亮，吕生华，侯明明，弓瑞

陕西科技大学资源与环境学院，陕西西安 710021

出版日期:2011-12-05 发布日期:2011-12-05

Synthesis and characterization of graft copolymer of degraded starch and acrylamide initiated by immobilized HRP/H2O2/ACAC catalyzed system

YAN Xiaoliang，Lü Shenghua，HOU Mingming，GONG Rui

College of Resource and Environment，Shaanxi University of Science & Technology，Xi’an 710021，Shannxi，China

Online:2011-12-05 Published:2011-12-05

摘要/Abstract

摘要：

以壳聚糖为载体、戊二醛为交联剂制备固定化辣根过氧化物酶（HRP），采用固定化HRP/H₂O₂/乙酰丙酮（ACAC）酶促体系制备降解淀粉-丙烯酰胺接枝共聚物（St-g-PAM），通过红外光谱、紫外光谱、核磁共振和电镜扫描等手段对接枝共聚产物进行了结构分析。结果表明，丙烯酰胺成功接枝在降解淀粉上。共聚物用做皮革复鞣剂进行了应用实验，应用结果表明复鞣后的革柔软、纤维分散好和选择填充性强。

Abstract:

Horseradish peroxidase（HRP）was immobilized on the surface of chitosan via glutaraldehyde crosslinking. Then a graft copolymer of degraded starch and acrylamide was synthesized by the initiation of enzyme catalysis system composed of chitosan-immobilized HRP/H₂O₂/acetylacetone（ACAC）. The structure of the copolymer was analyzed by FTIR，UV，NMR and SEM. The results indicated that polyacrylamide（PAM）was successfully grafted onto the degraded starch. Then，the copolymer was applied as a retanning agent. The applied results showed that the retanned leather had the merits of softness，good dispersion of fiber and strong selecting filling properties.

闫小亮，吕生华，侯明明，弓瑞. 固定化HRP/H2O2/ACAC酶促体系引发下降解淀粉接枝丙烯酰胺共聚物的合成与表征[J]. 化工进展.

YAN Xiaoliang，Lü Shenghua，HOU Mingming，GONG Rui. Synthesis and characterization of graft copolymer of degraded starch and acrylamide initiated by immobilized HRP/H2O2/ACAC catalyzed system[J]. Chemical Industry and Engineering Progree.