Abstract:

For preparative applications in asymmetric reduction with alcohol dehydrogenase，three issues have to be considered：An appropriate enzyme，an efficient coenzyme-regenerating method and a suitable in vitro evolution technique. Enzyme is the source of effective biocatalyst. The native or recombinational alcohol dehydrogenases from Rhodococcus sp.，Lactobacillus sp.，Leifsonia sp. and thermophilic microbiology，and their catalysis were analyzed. The advantages and disadvantages of coenzyme-regeneration methods were discussed. The efficient coenzyme-regeneration can decrease costs for large-scale application. In vitro evolution technique can improve the activity，stability and selectivity of target enzyme. The final aim is to establish effective and green ways to obtain chiral drug intermediates by enzymatic synthesis.