Abstract: The fermentation conditions of endophytic fungus Fusarium mairei UH23 were investigated for taxol production. The results showed that an inoculation volume of 5% into the 60 mL/250 mL Erlenmeyer flask at 26 ℃ and initial pH 7.0 could reach the maximum taxol production after 12 days of incubation. Glucose and ammonium nitrate were selected as the best carbon and nitrogen sources in the further study. The taxol yield under the optimized conditions reached (286.4±20.3) μg/L，an increase by 10.2%.