Abstract: Chitosan beads were prepared by the dripping granulation method，using chitosan (CS) as the raw material，sodium tripolyphosphate (TPP) and epichlorohydrin (ECH) as the cross-linking reagent. The adsorption properties of the chitosan beads for two kinds of acidic dyes，acid bordeaux B(ABB) and nylosan yellow N-3RL(NYN) from water was investigated. Under the initial concentration of dye waste water 100 mg / L and volume 50 mL，the optimum adsorption conditions of chitosan beads for the two dye waste waters were obtained as follows：particle size of chitosan beads 0.5mm，dosage 20.0mg，contact time 16 h，pH value 3， temperature 20℃ for ABB，30—50℃ for NYN，oscillating rate 150r/min. Under the optimum adsorption conditions，the adsorption process of ABB and NYN fitted well with both Freundlich model and Langmuir model，and the maximum adsorption capacity for ABB and NYN were 714.29 mg/g and 769.23 mg/g respectively，which exceeded the maximum adsorption capacity by non-bead chitosan 443.99 mg/g，102.53 mg/g respectively. The saturated chitosan beads were desorbed by 0.01mol/L NaOH，the regeneration rate were 99.08%，99.35% for each dye，and the beads could be used repeatedly which would reduce the decoloration cost.