Abstract: A novel aminated chitosan derivative was prepared from chitosan through cross-linking, hydroxylpropyl-chloridization and amination. The effect of different amination agents and the amount of amination agent on the amino group content of the aminated chitosan derivative and the adsorption characteristics for flavones by the aminated chitosan derivative were investigated. The structure of the micorsphere was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). The results indicated that, the properties of the novel aminated chitosan derivative were improved significantly as compared with chitosan.